rabbit anti cripto 1  (Cell Signaling Technology Inc)

Journal: Oncology Letters

Article Title: Translational significance of Nodal, Cripto-1 and Notch4 in adult nevi

doi: 10.3892/ol.2016.4755

Figure Lengend Snippet: Immunohistochemistry results. Representative images of immunohistochemistry staining results showing weak and strong staining for Nodal, Cripto-1 and Notch4 in nevi. IS, index score.

Article Snippet: Briefly, following antigen retrieval and blocking steps, sections were incubated in primary antibody for 60 min [mouse monoclonal anti-Nodal (1:200 dilution; cat no. ab55676; Abcam, Cambridge, MA, USA); rabbit polyclonal anti-Notch4 (1:80 dilution; cat no. sc-5594; Santa Cruz Biotechnology, Inc., Dallas, TX, USA); rabbit polyclonal anti-Cripto-1 (1:1500 dilution; cat no. 600-401-997; Rockland Scientific International Inc., Limerick, PA, USA)], followed by ready-to-use anti-rabbit (cat no. GR602H) or anti-mouse (cat no. GM601H) biotinylated secondary antibody (Biocare Medical Inc., Concord, CA, USA), and then streptavidin-horseradish peroxidase (ThermoFisher Scientific Inc., Waltham, MA, USA).

Techniques: Immunohistochemistry, Staining

Journal: Oncology Letters

Article Title: Translational significance of Nodal, Cripto-1 and Notch4 in adult nevi

doi: 10.3892/ol.2016.4755

Figure Lengend Snippet: Immunohistochemistry IS. Histograms representative of mean immunohistochemistry IS for Nodal, Cripto-1 and Notch4 staining in nevi from patients with or without a Hx of melanoma and in cutaneous and metastatic melanoma (*P<0.05; t -test). IS, index score; Hx, history.

Article Snippet: Briefly, following antigen retrieval and blocking steps, sections were incubated in primary antibody for 60 min [mouse monoclonal anti-Nodal (1:200 dilution; cat no. ab55676; Abcam, Cambridge, MA, USA); rabbit polyclonal anti-Notch4 (1:80 dilution; cat no. sc-5594; Santa Cruz Biotechnology, Inc., Dallas, TX, USA); rabbit polyclonal anti-Cripto-1 (1:1500 dilution; cat no. 600-401-997; Rockland Scientific International Inc., Limerick, PA, USA)], followed by ready-to-use anti-rabbit (cat no. GR602H) or anti-mouse (cat no. GM601H) biotinylated secondary antibody (Biocare Medical Inc., Concord, CA, USA), and then streptavidin-horseradish peroxidase (ThermoFisher Scientific Inc., Waltham, MA, USA).

Techniques: Immunohistochemistry, Staining

Journal: Oncology Letters

Article Title: Translational significance of Nodal, Cripto-1 and Notch4 in adult nevi

doi: 10.3892/ol.2016.4755

Figure Lengend Snippet: Median IS. Box plots representative of the distribution and median value of immunohistochemistry IS for Nodal, Cripto-1 and Notch4 staining in adult nevi (*P<0.05; median test). IS, index score; Hx, history.

Article Snippet: Briefly, following antigen retrieval and blocking steps, sections were incubated in primary antibody for 60 min [mouse monoclonal anti-Nodal (1:200 dilution; cat no. ab55676; Abcam, Cambridge, MA, USA); rabbit polyclonal anti-Notch4 (1:80 dilution; cat no. sc-5594; Santa Cruz Biotechnology, Inc., Dallas, TX, USA); rabbit polyclonal anti-Cripto-1 (1:1500 dilution; cat no. 600-401-997; Rockland Scientific International Inc., Limerick, PA, USA)], followed by ready-to-use anti-rabbit (cat no. GR602H) or anti-mouse (cat no. GM601H) biotinylated secondary antibody (Biocare Medical Inc., Concord, CA, USA), and then streptavidin-horseradish peroxidase (ThermoFisher Scientific Inc., Waltham, MA, USA).

Techniques: Immunohistochemistry, Staining

Journal: Oncology Letters

Article Title: Translational significance of Nodal, Cripto-1 and Notch4 in adult nevi

doi: 10.3892/ol.2016.4755

Figure Lengend Snippet: Correlation analyses. Linear correlation analysis between Nodal, Cripto-1 and Notch4 immunohistochmistry IS in nevi from patients with or without melanoma Hx. IS, index score; Hx, history.

Article Snippet: Briefly, following antigen retrieval and blocking steps, sections were incubated in primary antibody for 60 min [mouse monoclonal anti-Nodal (1:200 dilution; cat no. ab55676; Abcam, Cambridge, MA, USA); rabbit polyclonal anti-Notch4 (1:80 dilution; cat no. sc-5594; Santa Cruz Biotechnology, Inc., Dallas, TX, USA); rabbit polyclonal anti-Cripto-1 (1:1500 dilution; cat no. 600-401-997; Rockland Scientific International Inc., Limerick, PA, USA)], followed by ready-to-use anti-rabbit (cat no. GR602H) or anti-mouse (cat no. GM601H) biotinylated secondary antibody (Biocare Medical Inc., Concord, CA, USA), and then streptavidin-horseradish peroxidase (ThermoFisher Scientific Inc., Waltham, MA, USA).

Techniques: